[1]程俊文,贺亮,魏海龙,等.超声波协同复合酶法提取桑黄多糖工艺优化[J].浙江林业科技,2017,37(04):33-38.[doi:10.3969/j.issn.1001-3776.2017.04.006]
　CHENG Jun-wen,HE Liang,WEI Hai-long,et al.Ultrasonic-assisted Enzymatic Method Extraction of Polysaccharide from Inonotus sanghuang[J].Journal of Zhejiang Forestry Science and Technology,2017,37(04):33-38.[doi:10.3969/j.issn.1001-3776.2017.04.006]
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超声波协同复合酶法提取桑黄多糖工艺优化()
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《浙江林业科技》[ISSN:1001-3776/CN:33-1112/S]

卷:
37
期数:
2017年04期
页码:
33-38
栏目:
出版日期:
2017-09-30

文章信息/Info

Title:
Ultrasonic-assisted Enzymatic Method Extraction of Polysaccharide from Inonotus sanghuang
作者:
程俊文1; 贺亮1; 魏海龙1; 宋吉玲2; 胡传久1; 邹景泉1; 李海波1; 蒋云鹤1
1. 浙江省林业科学研究院 浙江省森林资源生物与化学利用重点实验室，浙江杭州 310023；2. 杭州市农业科学研究院， 浙江 杭州 310024
Author(s):
CHENG Jun-wen1; HE Liang1; WEI Hai-long1; SONG Ji-ling2; HU Chuan-jiu1; ZOU Jing-quan1; LI Hai-bo1; JIANG Yun-he1
1. Zhejiang Academy of Forestry, Key Laboratory of Biological and Chemical Utilization of Forest Resources, Hangzhou 310023, China; 2. Hangzhou Academy of Agricultural Sciences of Zhejiang, Hangzhou 310024, China
关键词:
桑黄; 多糖; 超声波; 复合酶; 响应面
Keywords:
Inonotus sanghuang;  polysaccharide;  ultrasonic;  compound enzyme;  response surface methodology
分类号:
TQ461
DOI:
10.3969/j.issn.1001-3776.2017.04.006
文献标志码:
A
摘要:
在单因素试验的基础上，通过Box-Benhnken组合设计和响应曲面分析法优化桑黄Inonotus sanghuang子实体中多糖的超声波协同复合酶法提取工艺条件。在前期预实验的基础上，采用纤维素酶、果胶酶和蛋白酶按2:1:1组成复合酶对桑黄子实体进行酶解处理，添加量为2%，温度50℃，pH 6.5，在水溶液中酶解1 h。然后对工艺中的超声波功率、超声时间、液料比进行优化。响应面分析结果表明，桑黄子实体中多糖的最佳提取条件为：超声功率360.6 W，液料比32.5:1，超声时间32.7 min，多糖得率可得3.31%，与理论值3.46%基本吻合。
Abstract:
Extraction technology of polysaccharide from Inonotus sanghuang was optimized by ultrasonic-assisted enzymatic method and response surface analysis. Based on the results of single factor experiment, enzymolysis of I. sanghuang sporcarp was carried out with 2% of complex phosphoesterasum (ratio of cellulose, pectinase, protease of 2:1:1) at 50℃ under pH of 6.5 for 1 hour. Then optimization of extraction technology was implemented on ultrasonic power, ultrasound time and ratio of liquid and material. The response surface analysis demonstrated that polusaccharide yield topped 3.31% under condition as follows: ultrasonic power 360.6W, liquid/material ratio 32.5:1, ultrasound treatment time 32.7minutes, similar to theoretical value of 3.46%.

参考文献/References:

[1] 吴声华，黄冠中，陈愉萍，等. 桑黄的分类及开发前景[J].菌物研究，2016，14（4）：187－200.
[2] Yan J K，Wang Y Y，Ma H L，et al. Ultrasonic effects on the degradation kinetics, preliminary characterization and antioxidant activities of polysaccharides from Phellinus linteus mycelia[J]. Carbohydr Polym，2016，29，251－257.
[3] Zhang Z F，Lu G Y，Song T T，et al. Comparison of the preliminary characterizations and antioxidant properties of polysaccharides obtained from Phellinus baumii growth on different culture substrates [J]. Carbohydr Polym，2015，397－399.
[4] Kim G Y，Kim S H，Hwang S Y，et al. Oral administration of proteoglycan isolated from Phellinus linteus in the prevention and treatment of collagen-induced arthritis in mice[J]. Biol Pharm Bull，2003，26（6）：823－83l.
[5] 王洪伟，崔崇士. 南瓜多糖复合酶法提取及纯化的研究平[J]. 食品科学，2007，28（8）：247－249.
[6] 赵玉红，王静，金秀明. 超声波辅助酶法提取榛子壳色素工艺条件的研究[J]. 中国调味品，2010，35（4）：110－114.
[7] 徐志祥，李刚，李宝建. 苯酚－硫酸法测定灵芝多糖含量的条件研究[J]. 食用菌，2000，22（3）：6－7.
[8] 张成军. 实验设计与数据处理[Ｍ]. 北京：化学工业出版社，2009.
[9] Jin Q L，Zhang Z F，Lu G Y，et al. Antioxidant and DNA damage protecting potentials of polysaccharideextracted from Phellinus baumii using a delignification method[J]. Carbohydr Polym，2016，152，575－582.

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备注/Memo

备注/Memo:
收稿日期：2017-03-09；修回日期：2017-06-23
基金项目： 浙江省科技厅院所专项（2014F50018）；国家自然科学基金项目（31501815）；浙江省农业（食用菌）新品种选育项目（2016C02057-8） 和杭州市科委种子种苗专项（20150932H07）的部分研究内容
作者简介：程俊文，硕士，副研究员，从事食用药用菌活性成分加工研究；E-mail：jwchengzj@163.com。
通信作者：邹景泉，工程师，从事森林资源研究；E-mail：422046791@qq.com。
更新日期/Last Update: 2017-08-30