[1]石从广,高燕会,柳新红,等.3种安息香属植物的根尖染色体核型分析[J].浙江林业科技,2017,37(03):23-27.[doi:10.3969/j.issn.1001-3776.2017.03.004]
 SHI Cong-guang,GAO Yan-hui,LIU Xin-hong,et al.Karyotype Analysis of Root Chromosome in Three Species of Styrax[J].Journal of Zhejiang Forestry Science and Technology,2017,37(03):23-27.[doi:10.3969/j.issn.1001-3776.2017.03.004]
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3种安息香属植物的根尖染色体核型分析()
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《浙江林业科技》[ISSN:1001-3776/CN:33-1112/S]

卷:
37
期数:
2017年03期
页码:
23-27
栏目:
出版日期:
2017-07-31

文章信息/Info

Title:
Karyotype Analysis of Root Chromosome in Three Species of Styrax
作者:
石从广1高燕会2柳新红1李因刚1杨少宗1蒋冬月1朱光权1
1. 浙江省林业科学研究院,浙江杭州 310023;2. 浙江农林大学,浙江 临安311300
Author(s):
SHI Cong-guang1GAO Yan-hui2LIU Xin-hong1LI Yin-gang1YANG Shao-zong1JIANG Dong-yue1ZHU Guang-quan1
1. Zhejiang Academy of Forestry, Hangzhou 310023, China; 2. Zhejiang A & F University, Lin’an 311300, China
关键词:
安息香属染色体核型分析二倍体进化
Keywords:
Styrax chromosome karyotype analysis diploid evolution
分类号:
S722.3
DOI:
10.3969/j.issn.1001-3776.2017.03.004
文献标志码:
A
摘要:
2013年9月和10月,在浙江省杭州市采集芬芳安息香Styrax odoratissimus,越南安息香S. tonkinensis和赛山梅S. confusus种子,砂藏至翌年发芽,待根长至3 ~ 5 cm时,对3种安息香属植物的根尖进行染色体制片和核型分析。结果显示,3树种均为二倍体,体细胞染色体数均为2n﹦2x﹦16。芬芳安息香核型公式为2n﹦16﹦10 m+6 sm(2 sat),核不对称系数为58.20%,染色体核型为2A型;越南安息香核型公式为2n﹦16﹦12 m(1 sat)+4 sm (2 sat),核不对称系数为53.27% ,染色体核型为1A型;赛山梅染色体核型公式为2n﹦16﹦12 m(1sat)+4 sm,核不对称系数为56.54%,染色体核型为1A型。表明越南安息香和赛山梅核型较为类似,亲缘关系较近,与芬芳安息香之间差别较大,芬芳安息香比越南安息香和赛山梅进化程度较高。3种安息香属植物染色体核型均为A型,表明其进化地位较低,植物较为原始。
Abstract:
Seeds of Styrax odoratissimus, S. tonkinensis and S. confusus were collected on September and October of 2013 in Hangzhou, Zhejiang province. They were treated by sand storage until the next spring. When seeds germinated and root had 3-5 cm, the apices of three species were used for chromosome sectioning and karyotype analysis. The results showed that the tested species were diploid, and their genome chromosome number was 2n=2x=16. Karyogram of S. odoratissimus was 2n=16=10 m+6 sm (sat), nuclear asymmetry index was 58.20%, and karyotype was 2A. Karyogram of S. tonkinensis was 2n=16=12m(1sat)+4sm(2sat), nuclear asymmetry index was 53.09%, andt karyotype was 1A. Karyogram of S. confusus was 2n=16=12 m(1sat)1+4 sm, nuclear asymmetry index was 56.54%, and the karyotype was 1A. The test concluded that karyotypes of S. tonkinensis and S. confusus were similar, but different with that of S. odoratissimus, indicating that the chromosome evolution of three species was low, and they were primitive.

参考文献/References:

[1] 张程. 中国安息香科植物种质资源及研究进展[J]. 江西林业科技,2010年,(6):42-47.
[2] 中国科学院植物研究所.中国高等植物图鉴第三册[M]. 北京:科学出版社,1985,338.
[3] 吴君,李因刚,罗修宝,等. 白花树种子生物学特性[J]. 浙江农林大学学报,2014,31(1):9-13.
[4] 吴君,李因刚,柳新红,等. 越南安息香大小孢子发生和雌雄配子体发育[J]. 浙江林业科技,2014,34(1):47-51.
[5] 吴君,李因刚,柳新红,等. 白花树花粉生活力检测方法与贮藏特性研究[J]. 林业科学研究,2014,27(1):017-023.
[6] 李因刚,柳新红,赵勋,等. 我国越南安息香的地理分布及其气候特征[J]. 西南林业大学学报,2011,31(1):5-10.
[7] 陈涛,陈忠毅. 安息香科植物地理分布研究[J]. 植物研究,1996,35(1):97-99.
[8] 柳新红,李因刚,赵勋,等. 白花树天然群体表型多样性研究[J]. 林业科学研究2011,24(6):694-700.
[9] 谢建秋,柳新红,王军峰,等.东京野茉莉引种试验初报[J]. 浙江林业科技,2006,26(4):33-35.
[10] 顾雁蕾,叶兴乾,柳新红.野茉莉种仁与种子油的营养成分及理化特性研究[J]. 食品工业科技,2010,12(31):328-329.
[11] 肖复明,曾志光,杨桦,等. 东京野茉莉种子油营养成分研究[J]. 天然产物研究与开发,2005,17(3):344-345.
[12] 张亮亮,柳新红,林新春. 白花树组织培养技术研究[J]. 浙江林业科技,2013,33(3):16-19.
[13] 石从广,朱光权,柳新红,等. 施肥对白花树各生长性状的影响[J]. 浙江林业科技,2013,33(3):9-15.
[14] 石从广,李因刚,朱光权,等. 白花树种子成熟期含油率和脂肪酸变化规律探讨[J]. 浙江农林大学学报,2013,30(3):372-378.
[15] 石从广,朱光权,黄海志,等. 白花树种子油精炼及其毒理学初步评价[J]. 中国食品学报,2014,14(5):194-201.
[16] 刘光斌,黄长干,刘苑秋,等. 东京野茉莉油的提取及其制备生物柴油的初步研究[J]. 江西农业大学学报,2007,29(4):685-689.
[17] 陈洁,聂小安,刘振兴,等. 环氧白花果油基脂肪酸甲酯的无羧酸法制备工艺[J]. 中国油脂,2014(7):69-72.
[18] 鲍甫成,江泽慧. 短周期工业用材林木材性质研究[J]. 世界林业研究,1995,(8),50-56.
[19] 骆显春,杨桦,曾志光,等. 东京野茉莉木材性质分析与利用[J]. 江西农业大学学报,2007,29(1):77-80.
[20] LIU Z X,NIE X A,WANG Y G. Biodiesel Preparation from Styrax confusus Hemsl. Oil Catalyzed by Magnetic Catalyst S2O82--ZrO2/Fe3O4[C], International Conference on Advances in Energy & Environment Science,2013,805-806,247-250.
[21] Levan A,Fredga K,Sandberg A A. Nomenclature for centromerie position on chromosome [J]. Hereditas,1964,52:201-220.
[22] 李懋学,陈瑞阳.关于植物核型分析的标准化问题[J]. 武汉植物学研究,1985,3(4):297-302.
[23] Stebbins G L. Chromosome evolution in higher plants [M]. London:Edward Arnold Ltd,1971:87-123.
[24] Arano H. Cytological studies in subfamily Carduoideae (Composite) of Japan IX[J]. Bot Mag Tokyo,1963,76:419-427.

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备注/Memo

备注/Memo:
收稿日期:2016-11-16;修回日期:2017-02-21
基金项目:本稿件受浙江省农业公益性研究项目“安息香属优质能源树种选择与开发利用”资助(2012C22097)
作者简介:石从广,博士,副研究员,从事林木遗传改良研究;E-mai:363928874@qq.com。通信作者:朱光权,教授级高工,从事植物分类学研究;E-mail:2554396001@qq.com。
更新日期/Last Update: 2017-08-06