[1]李新伟,李婷,张镇梁,等.不同牡丹品种花粉的可溶性糖、可溶性蛋白含量和抗氧化酶活性分析[J].浙江林业科技,2023,43(04):57-62.[doi:10.3969/j.issn.1001-3776.2023.04.008]
 LI Xinwei,LI Ting,ZHANG Zhenliang,et al.Analysis of Soluble Sugar, Soluble Protein Content and Antioxidant Enzyme Activity in Pollen of Different Tree Peony Varieties[J].Journal of Zhejiang Forestry Science and Technology,2023,43(04):57-62.[doi:10.3969/j.issn.1001-3776.2023.04.008]
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不同牡丹品种花粉的可溶性糖、可溶性蛋白含量和抗氧化酶活性分析()
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《浙江林业科技》[ISSN:1001-3776/CN:33-1112/S]

卷:
43
期数:
2023年04期
页码:
57-62
栏目:
出版日期:
2023-07-21

文章信息/Info

Title:
Analysis of Soluble Sugar, Soluble Protein Content and Antioxidant Enzyme Activity in Pollen of Different Tree Peony Varieties
文章编号:
1001-3776(2023)04-0057-06
作者:
李新伟李婷张镇梁张彤张峥乔琦侯小改
河南科技大学 农学院/牡丹学院,河南 洛阳 471000
Author(s):
LI XinweiLI TingZHANG ZhenliangZHANG TongZHANG ZhengQIAO QiHOU Xiaogai
Agricultural College/Peony College, Henan University of Science and Technology, Luoyang 471023, China
关键词:
牡丹花粉可溶性糖可溶性蛋白抗氧化酶
Keywords:
Paeonia×suffruticosa pollen soluble sugar soluble protein antioxidant enzyme
分类号:
中国分类号:S567.1+5
DOI:
10.3969/j.issn.1001-3776.2023.04.008
文献标志码:
A
摘要:
以早花、中花、晚花16个牡丹Paeoniasuffruticosa栽培品种的花粉为材料,采用蒽酮比色法和凯氏定氮法分别测定其可溶性糖和可溶性蛋白含量,并比较不同温度下花粉的超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性变化。结果表明,不同花期的牡丹品种花粉中的可溶性糖和可溶性蛋白含量存在显著差异(P<0.05),以中花品种花粉中的可溶性蛋白含量较高,晚花品种花粉中的可溶性糖含量较高,其中,中花品种‘肉芙蓉’花粉中的可溶性蛋白含量最高,达1.35mg·g-1,晚花品种‘五大洲’花粉中的可溶性糖含量最高,达74.19mg·g-1;不同温度贮藏24h后,各牡丹品种花粉的抗氧化酶活性不同,大部分品种花粉的SOD和POD活性随着温度的降低逐渐增加,而CAT活性的变化趋势则复杂多变。由于大部分品种的SOD和POD酶活性在4℃时较高,因此推荐4℃为牡丹花丝茶的常规保存温度。
Abstract:
During April and May 2020, pollen of 16 cultivars of Paeonia×suffruticosa was collected from Luoyang, Henan province to determine soluble sugar and soluble protein content, as well as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities of pollen stored under different temperatures. The results showed that there were significant differences among cultivars with different flowering stages, and the mid-flowering cultivars were dominant in soluble protein and the late-flowering ones in soluble sugar content. The soluble protein content of the mid-flowering cultivar P.×suffruticosa‘Roufurong’ was the highest, 1.35 mg/g and the late-flowering one P.×suffruticosa‘Wudazhou’ was the highest, 74.19 mg/g. After 24 hours of storage under different temperatures, the antioxidant enzyme activities of each cultivar were different. The activities of SOD and POD enzymes in most cultivars increased gradually with the decrease of temperature, while the change trend of CAT enzyme activity was more complex. SOD and POD activities of most cultivars were Relative higher at 4 ℃.

参考文献/References:

[1] 中国牡丹全书编纂委员会. 中国牡丹全书(上)[M]. 北京:中国科学技术出版社,2002:87-95.
[2] 孙静,姜宇,陶俊. 牡丹与芍药的食用研究进展[J]. 江苏农业科学,2018,46(11):10-14.
[3] 李运,邱国玉,石晓峰,等. 甘肃紫斑牡丹花粉中脂肪酸的分析研究[J]. 中国现代应用药学,2017,34(06):849-853.
[4] 张孟琴,孙亚真,范一霖. 牡丹花粉营养成分评价及相关性分析[J]. 食品研究与开发,2018,39(15):154-160.
[5] 温可馨,司冰. 中原牡丹不同品种花粉活力及授粉特性研究[J]. 现代化农业,2017(10):31-33.
[6] 李志,李琳,石晓峰,等. 牡丹花粉口含片的质量标准研究[J]. 中国药房,2019,30(09):1246-1250.
[7] 王斌利,张莉霞,石晓峰,等. 紫斑牡丹花粉不同浓度乙醇提取物抗氧化能力研究[J]. 中国中医药信息杂志,2018,25(02):65-68.
[8] 王士泉. 四川牡丹花粉生活力测定方法的比较[J]. 热带作物学报,2022,41(01):57-62.
[9] 张治安,张美善,蔚荣海,等. 植物生理学实验[M]. 北京:中国农业科学技术出版社,2003:132-134.
[10] 杨兰芳,曾巧,李海波,等. 紫外分光光度法测定土壤过氧化氢酶活性[J]. 土壤通报,2011,42(01):207-210.
[11] 刘培秀,赵美萱,杨梅,等. 西兰花粉营养成分及理化性质研究[J]. 食品工业科技,2022,43(11):326-333.
[12] 何得平. 临夏油用紫斑牡丹花粉营养成分分析[J]. 林业科技通讯,2017(03):57-58.
[13] 游玉明,杨帆,熊运海. 牡丹花的综合利用与开发前景[J]. 北方园艺,2011(01):67-69
[14] 李志,李琳,石晓峰,等. 牡丹花粉口含片的制备工艺及抗氧化活性[J]. 甘肃农业大学学报,2020,55(01):177-185.
[15] 张孟琴,孙亚真,范一霖. 牡丹花粉营养成分评价及相关性分析[J]. 食品研究与开发,2018,39(15):154-160.
[16] 贺春玲,徐珊珊,张淑霞,等. 9种牡丹花粉的蛋白质和矿物元素含量分析[J]. 核农学报,2015,29(11):2158-2164.
[17] 潘琦,宋祥甫,邹国燕,等. 不同温度对沉水植物保护酶活性的影响[J]. 生态环境学报,2009,18(05):1881-1886.
[18] ALI M B,HAHN E J,PAEK K Y. Effects of temperature on oxidative stress defense systems, lipid peroxidation and lipoxygenase activity in Phalaenopsis[J]. Plant Physiol Biochem,2005,43(3):213-223.
[19] 陈旭微,杨玲,章艺,等. 10℃低温对绿豆和豌豆下胚轴细胞一些抗氧化酶活性和超微结构的影响[J]. 植物生理与分子生物学学报,2005, 31(5):539-544.
[20] YILDIZ M,TERZI H,BING?L N. Protective role of hydrogen peroxide pretreatment on defense systems and BnMP1 gene expression in Cr(VI)-stressed canola seedlings[J]. Ecotoxicology,2013,22(8):1303-1312.
[21] ALI B,TAO Q,ZHOU Y F,et al. 5-Aminolevolinic acid mitigates the cadmium-induced changes in Brassica napus as revealed by the biochemical and ultrastructural evaluation of roots[J]. Ecotoxicol Environ Saf,2013,92:271-280.
[22] QIU Z,LI J T,ZHANG J,et al. Microwave pretreatment can enhance tolerance of wheat seedlings to CdCl2 stress[J]. Ecotoxicol Environ Saf, 2011,74(4):820-825.
[23] ARORA P,BHARDWAJ R,KUMAR KANWAR M. 24-epibrassinolide induced antioxidative defense system of Brassica juncea L. under Zn metal stress[J]. Physiol Molec Biol Plant,2010,16(3):285-293.
[24] SHI H,YE T,CHAN Z. Exogenous application of hydrogen sulfide donor sodium hydrosulfide enhanced multiple abiotic stress tolerance in bermudagrass (Cynodon dactylon (L). Pers.[J]. Plant Physiol Biochem,2013,71:226-234.
[25] WANG S,LIANG D,LI C,et al. Influence of drought stress on the cellular ultrastructure and antioxidant system in leaves of drought-tolerant and drought-sensitive apple rootstocks[J]. Plant Physiol Biochem,2012,51:81-89.
[26] 刘强,王庆成,徐静,等. 外源亚精胺和精胺对NaHCO3胁迫下南蛇藤抗氧化系统的影响[J]. 应用生态学报,2009,20(3):549-554.
[27] ZHANG S,ZHANG H,QIN R,et al. Cadmium induction of lipid peroxidation and effects on root tip cells and antioxidant enzyme activities in Vicia faba L.[J]. Ecotoxicology,2009,18(7):814-823.
[28] LIU D Y,LI Y,CHU L,et al. Study of the tolerance of Hippochaete ramosissimum to Cu stress[J]. Sci Chin Ser,C Life Sci,2005,48(1):150-155.
[29] 谭健晖. 贮藏温度和时间对马尾松花粉保护酶活性及萌发率的影响[J]. 林业科学,2011,47(9):28-32.

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备注/Memo

备注/Memo:
收稿日期:2023-01-17;修回日期:2023-05-12
作者简介:李新伟,硕士,从事植物学研究; E-mail:929490247@qq.com。
通信作者:乔琦,教授,博士,从事植物学研究;E-mail:nxyqiao@163.com。
更新日期/Last Update: 2023-07-20