[1]李修鹏,沈登锋,徐沁怡,等.不同贮藏方法对华顶杜鹃花粉活力的影响[J].浙江林业科技,2019,39(5):72-76.[doi:10.3969/j.issn.1001-3776.2019.05.011]
 LI Xiu-peng,SHEN Deng-feng,XU Qin-yi,et al.Effects on Pollen Viabilities of Rhododendron huadingense with Different Storage Methods[J].Journal of Zhejiang Forestry Science and Technology,2019,39(5):72-76.[doi:10.3969/j.issn.1001-3776.2019.05.011]
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不同贮藏方法对华顶杜鹃花粉活力的影响()
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《浙江林业科技》[ISSN:1001-3776/CN:33-1112/S]

卷:
39
期数:
2019年第5期
页码:
72-76
栏目:
试验简报
出版日期:
2019-11-30

文章信息/Info

Title:
Effects on Pollen Viabilities of Rhododendron huadingense with Different Storage Methods
文章编号:
1001-3776(2019)05-0072-05
作者:
李修鹏1沈登锋2徐沁怡1章建红2何立平3李东宾3余敏芬3沈波1
1.宁波市林特科技推广中心,浙江宁波 315012;2.宁波市农业科学研究院,浙江宁波315040;3.宁波市林场,浙江余姚 315440
Author(s):
LI Xiu-peng1SHEN Deng-feng2XU Qin-yi1ZHANG Jian-hong2HE Li-ping3LI Dong-bin3YU Min-feng3SHEN Bo1
1. Ningbo Technology Extension Center for Forestry and Specialty Forest Products, Ningbo 315012, Zhejiang, China;2. Ningbo Agricultural Science Academy, Ningbo 315040, Zhejiang, China;3. Ningbo forest farm,Yuyao 315440, Zhejiang, China
关键词:
华顶杜鹃花粉活力贮藏方法
Keywords:
Rhododendron huadingense pollen viability storage condition
分类号:
S685.21
DOI:
10.3969/j.issn.1001-3776.2019.05.011
文献标志码:
A
摘要:
研究了华顶杜鹃Rhododendron huadingense花粉活力水平及不同贮藏方法对其活力的影响。结果表明,新鲜华顶杜鹃花粉的萌发率高达98.1%;室温存放的新鲜花粉在3d内可保持较高的活力,3 d后其活力下降迅速;4℃贮藏7 d后的花粉萌发率仍保持在85.5%的较高水平,贮藏15 d时的萌发率仍高于50%,可作为华顶杜鹃花粉的短期(15 d内)保存方法;-20℃贮藏40 d后的花粉萌发率仍能达到87.4%,基本可满足实际需求;-80℃下贮藏60 d后萌发率依旧维持在95.2%,可用于华顶杜鹃花粉的长期保存。本研究结果说明花粉活力不是导致华顶杜鹃濒危的生物学原因。
Abstract:
The pollen viability of Rh.huadingense and the effects ofdifferent storage methodswere studied, the results showed that the germination rate of fresh pollens was as high as 98%, fresh pollens which were stored at room temperature (20℃) had a high activity in 3 days, then the activity of pollens declined rapidly, thegermination rate was about 85.5% when the pollens were stored at 4℃ for a week, after half a month ,the germination rate was up to 50%., It can be used as a short-term (within 15 days) preservation method for Rh. huadingense pollen. When stored at-20℃ for 40 days, the germination rate of pollens was up to 87.4%, while stored at-80℃ for 60 days,the germination ratecould remain at 95.2%, it could be used for the long-term preservation of the pollen.Our results indicate that pollen viability is not the biological reasonfor the endangered state of Rh. huadingense

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备注/Memo

备注/Memo:
收稿日期:2019-03-09;修回日期:2019-07-11
基金项目:宁波市农业重大科技专项(编号:2014C11002)
作者简介:李修鹏,正高级工程师,从事植物资源与森林培育研究;E-mail:lxpnb@163.com。通信作者:沈登锋,博士,工程师,从事林木遗传育种研究;E-mail:dengfeng028@163.com。
更新日期/Last Update: 2019-12-20