[1]肖程雨,李朋洋,郝小龙,等.加工及干燥方式对短小蛇根草活性成分的影响[J].浙江林业科技,2023,43(05):101-107.[doi:10.3969/j.issn.1001-3776.2023.05.014]
 XIAO Chengyu,LI Pengyang,HAO Xiaolong,et al.Effect of De-enzyme and Drying Methods on Active Elements of Ophiorrhiza pumila[J].Journal of Zhejiang Forestry Science and Technology,2023,43(05):101-107.[doi:10.3969/j.issn.1001-3776.2023.05.014]
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加工及干燥方式对短小蛇根草活性成分的影响()
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《浙江林业科技》[ISSN:1001-3776/CN:33-1112/S]

卷:
43
期数:
2023年05期
页码:
101-107
栏目:
研究简报
出版日期:
2023-09-28

文章信息/Info

Title:
Effect of De-enzyme and Drying Methods on Active Elements of Ophiorrhiza pumila
文章编号:
1001-3776(2023)05-0101-07
作者:
肖程雨12李朋洋2郝小龙2开国银12
1.浙江农林大学 食品与健康学院,浙江 杭州 311300;2.浙江中医药大学 药学院,浙江 杭州 311300
Author(s):
XIAO Chengyu12LI Pengyang2HAO Xiaolong2KAI Guoyin12
1.College of Food and Health, Zhejiang A & F University, Hangzhou 311300, China;2.College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 311300, China
关键词:
短小蛇根草杀青处理干燥方式喜树碱
Keywords:
Ophiorrhiza pumila de-enzyme drying methods camptothecin
分类号:
R284.2
DOI:
10.3969/j.issn.1001-3776.2023.05.014
文献标志码:
A
摘要:
为进一步推进短小蛇根草Ophiorrhizapumila在喜树碱产业上的应用,本研究利用高效液相色谱法、紫外可见分光光度法,分析了加工方法(杀青、不杀青)及干燥方式(晒干、阴干、烘干、真空冷冻干燥、真空干燥)对短小蛇根草中活性成分(喜树碱、总黄酮、粗多糖)的影响。结果表明,加工方法及干燥方式均对短小蛇根草活性成分影响较大,喜树碱在晒干后含量最高(1.90mg·g-1),总黄酮含量在真空干燥后含量最高(1.38mg·g-1),粗多糖在冷冻干燥后含量最高(50.01mg·g-1),均显著高于其他处理组(P<0.05);杀青会导致喜树碱含量普遍降低,总黄酮含量显著提高,粗多糖含量普遍降低。在实际生产中,以晒干作为短小蛇根草的首选干燥方式,干燥前不宜采取杀青处理。
Abstract:
In mid-September, Ophiorrhiza pumila were harvested in Jian’ou, Fujian province, and were treated by drying in sun, in shade, by baking, vacuum freeze drying and vacuum drying, as well as pretreated by de-enzyme then treated by different drying methods. High-performance liquid chromatography and UV-Vis spectrophotometry was used to determine active elements of dried O. pumila. The result demonstrated that the active elements of O. pumila were greatly influenced by the method of drying and de-enzyme. The content of camptothecin had the highest (1.90 mg/g) in dried O. pumila by sun drying, and the content of total flavonoids and crude polysaccharides was the highest in that by vacuum freezing drying, evidently higher than the other treatments(P<0.05). De-enzyme would decrease the content of camptothecin and crude polysaccharides, while significantly increase the content of total flavonoids. In practice, sun drying is the preferred method for O. pumila without de-enzyme before drying.

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备注/Memo

备注/Memo:
收稿日期:2023-03-17;修回日期:2023-07-03基金项目:国家自然科学基金面上项目(31571735);浙江省农业新品种选育重大科技专项(2021C02074);国家中医药管理局青年岐黄学者培养项目作者简介:肖程雨,硕士,从事中药材种子种苗工程研究;E-mail: chengyuxiao@stu.edu.cn。通信作者:开国银,教授,从事中药材种子种苗工程研究;E-mail: kaiguoyin@163.com。郝小龙,副研究员,从事中药材种子种苗工程研究;E-mail:haoxiaolong@zcmu.edu.cn。等[3-5]。药用植物短小蛇根草,对生长环境条件要求严苛,常为森林被破坏后第一批被消灭的植物,脆弱的野生资源导致其无法作为喜树碱的替代药源植物进行利用[6]。近年来,随着短小蛇根草栽培技术研究的逐步展开,水培型短小蛇根草可以很好地适应温室生长,并且生长速度快、喜树碱产量高,这使得短小蛇根草在未来有可能成为重要的喜树碱药源补充植物[7-10]
更新日期/Last Update: 2023-09-30