[1]陈海生,戴妍妍,陈少平,等.模拟氮沉降对山区水库消落带湿地土壤细菌群落结构的影响[J].浙江林业科技,2023,43(03):8-13.[doi:10.3969/j.issn.1001-3776.2023.03.002]
　CHEN Hai-sheng,DAI Yan-yan,CHEN Shao-ping,et al.Effect of Simulation Nitrogen Deposition on Rhizosphere Bacterial Diversity and Structure in Riparian Zone of Changtan Reservoir[J].Journal of Zhejiang Forestry Science and Technology,2023,43(03):8-13.[doi:10.3969/j.issn.1001-3776.2023.03.002]
点击复制

模拟氮沉降对山区水库消落带湿地土壤细菌群落结构的影响()
分享到：

《浙江林业科技》[ISSN:1001-3776/CN:33-1112/S]

卷:
43
期数:
2023年03期
页码:
8-13
栏目:
出版日期:
2023-05-20

文章信息/Info

Title:
Effect of Simulation Nitrogen Deposition on Rhizosphere Bacterial Diversity and Structure in Riparian Zone of Changtan Reservoir
文章编号:
1001-3776（2023）03-0008-06
作者:
陈海生1; 戴妍妍2; 陈少平3; 李振宇1; 徐蒋来4; 陈韬略5; 金思远1; 刘守平4
1.浙江同济科技职业学院，浙江 杭州 311231;2.浙江省台州市黄岩区北城街道办事处，浙江 台州 318020;3.浙江省余姚第二职业中学，浙江 余姚 315430;4.台州市农业科学研究院，浙江 临海 317000;5.浙江农林大学 数学与计算机学院，浙江 杭州 311300
Author(s):
CHEN Hai-sheng1; DAI Yan-yan2; CHEN Shao-ping3; LI Zhen-yu1; XU Jiang-lai4; CHEN Tao-lue5; JIN Si-yuan1; LIU Shou-ping4
1.Zhejiang Tongji Vocational College of Science and Technology, Hangzhou 311231, China;2.Huangyan Beicheng Subdistrict Office of Taizhou, Taizhou 318020, China;3.Yuyao Second Vocational Middle School, Zhejiang Yuyao 315430 ,China;4.Taizhou Academy of Agricultural Sciences of Zhejiang, Linhai 317000, China;5.School of Mathematics and Computer Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China
关键词:
氮沉降; 消落带湿地; 细菌群落; 高通量测序
Keywords:
nitrogen deposition;  riparian zone;  bacterial community;  high throughput sequencing
分类号:
S718.25+1.3；Q939.1
DOI:
10.3969/j.issn.1001-3776.2023.03.002
文献标志码:
A
摘要:
为了解氮沉降背景下水库消落带湿地土壤微生物群落结构对氮沉降浓度差异的响应，以浙江长潭水库消落带落羽杉Taxodiumdistichum种植区的湿地土壤为研究对象，设置了3个氮水平即低氮（NH4NO330kg·hm-2·a-1）、高氮（NH4NO360kg·hm-2·a-1）和对照（不施氮肥）的模拟氮沉降实验，采用高通量测序技术，分析水库消落带湿地土壤细菌群落对氮沉降浓度差异的响应。结果表明：在氮沉降增加条件下，水库消落带湿地土壤细菌的Shannon指数和Chao1指数在低氮条件下都是呈增加趋势，比对照分别增加了5.54%和13.29%，而在高氮条件下，其值呈降低趋势，分别比对照降低了4.41%和6.64%。氮沉降增加明显改变了消落带湿地土壤细菌群落结构，在门水平上，土壤酸杆菌门Acidobacteria相对丰度随着氮沉降的增加呈一直增加的趋势，土壤放线菌门Actinobacteria的相对丰度随着氮沉降的增加则呈一直下降的趋势；在属水平上，土壤芽单胞菌属Gemmatimonadetes和鞘脂单胞菌属Sphingomonas的相对丰度均随着氮沉降的增加呈先下降再升高的趋势。以上研究结果可为预测未来大气氮沉降对山地水库消落带湿地土壤微生物的影响以及湿地生态系统的变化提供理论参考。
Abstract:
In April 2020, experiments were conducted on simulation of different nitrogen deposition (0, 6 and 9 g/m2/year) at Taxodium distichum plantation in riparian zone of Changtan Reservoir, Zhejiang province. In April 2021, soil samples were collected and rhizsphere bacteria were analyzed by high throughput sequencing. The results indicated that 696 genera of 28 phylum and 87 classes and 168 orders of bacteria were detected. The Shannon and Chao 1 index of rhizosphere bacteria at treatment of 6 g/m2/year increased by 5.54% and 13.29% than that at the control (0 g/m2/year), while that at 9 g/m2/year decreased by 4.41% and 6.64% than that at the control. Simulation nitrogen deposition significantly changed the bacterial community structures. The relative abundance of Acidobacteria increased with nitrogen deposition, while that of Actinobacteria decreased at phylum level. The relative abundance of Gemmatimonadetes and Sphingomonas showed an initial decrease and subsequent increase with increasing nitrogen deposition at genus level.

参考文献/References:

[1] YUE K，FORNARA D A，YANG W Q，et al. Effects of three global change drivers on terrestrial C:N:P stoichiometry: a global synthesis[J]. Glob Chang Biol，2016，23（6）：24－50.
[2] LAMARQUE J F，KIEHL J T，BRASSEUR G P，et al. Assessing future nitrogen deposition and carbon cycle feedback using a multimodel approach: analysis of nitrogen deposition[J]. J Geophys Res：Atmospheres，2005，110（D19）：D19303.
[3] LIU X J，ZHANG Y，HAN W X，et al. Enhanced nitrogen deposition over China[J]. Nature，2013，494（7438）：459－462.
[4] LU C Q，TIAN H Q，LIU M L，et al. Effect of nitrogen deposition on China’s terrestrial carbon uptake in the context of multifactor environmental changes[J]. Ecol App，2012，22（1）：53－57.
[5] 段娜，李清河，多普增，等. 植物响应大气氮沉降研究进展[J]. 世界林业研究，2019，32（4）：6－11.
[6] 隋心，张荣涛，杨立宾，等. 模拟氮沉降对三江平原小叶章湿地土壤细菌多样性的影响[J]．草业科学，2016，33（4）：589－598．
[7] 徐润宏，谭梅，朱锦福，等. 高寒湿地土壤微生物多样性对氮沉降浓度差异的响应[J]. 生物学杂志，2021，38（6）：75－81.
[8] LI Y C，LI Z，LI Z W，et al. Variations of rhizosphere bacterial communities in tea ( Camellia sinensis L.) continuous cropping soil by high-throughput pyrosequencing approach[J]. J App Microbiol，2016，121（3）：787－799.
[9] BOLYEN E，RIDEOUT J R，DILLON M R，et al. Reproducible, interactive, scalable and extensible microbiome data science using QIIME2[J]. Nat Biotechnol，2019，37（8）：852－857.
[10] 魏志文，李韵雅，江威，等. 无锡地区常见树木根际土壤酸杆菌多样性[J]. 生态学杂志，2018，37（9）：2649－2656.
[11] FIERER N，BRADFORD M A，JACKSON R B. Toward and ecological classification of soil bacteria [J]. Ecology，2007，88（6）：1354－1364.
[12] 郭萍萍. 模拟氮沉降对土壤微生物多样性的影响[D]. 福州：福建农林大学，2015.
[13] 刘彩霞，焦如珍，董玉红，等. 杉木林土壤微生物区系对短期要模拟氮沉降的响应[J]. 林业科学研究，2015（2）：271－276.

相似文献/References:

[1]宋思睿,烟亚萍,宣丹娟,等.集约经营毛竹林土壤酶活性对模拟氮沉降的初期响应[J].浙江林业科技,2015,35(02):62.
[2]胡支舰,庾金武,陈健,等.氮沉降对低磷胁迫下毛竹实生幼苗生长及土壤化学特性的影响[J].浙江林业科技,2021,41(02):17.[doi:10.3969/j.issn.1001-3776.2021.02.003]
　HU Zhi-jian,YU Jin-wu,CHEN Jian,et al.Effect of Nitrogen Deposition on Growth of Phyllostachys edulis Seedlings and Soil Chemical Properties under Low Phosphorus Stress[J].Journal of Zhejiang Forestry Science and Technology,2021,41(03):17.[doi:10.3969/j.issn.1001-3776.2021.02.003]

备注/Memo

备注/Memo:
收稿日期：2022-12-05；修回日期：2023-03-02
基金项目：浙江省水利厅科技项目（RC1939）氮沉降增加背景下山地水库消落带湿地碳稳定性研究；浙江省科技厅项目（LGN18C030002）浙江省水库消落带落羽杉群落构建及植被恢复研究
作者简介：陈海生，博士，教授，从事土壤微生物研究；E-mail:haishch@126.com。
通信作者：刘守平，研究员，从事土壤微生物研究， E-mail:shpliu2005@126.com。
更新日期/Last Update: 2023-05-20