[1]蔡泱莲,黄宽官,黄俊浩,等.葱兰夜蛾幼虫肠道细菌群落结构与多样性分析[J].浙江林业科技,2019,39(02):7-14.[doi:10.3969/j.issn.1001-3776.2019.02.002]
 CAI Yang-lian,HUANG Kuan-guan,HUANG Jun-hao,et al.Analysis of Intestinal Community Structure and Diversity of Larvae of Brithys crini[J].Journal of Zhejiang Forestry Science and Technology,2019,39(02):7-14.[doi:10.3969/j.issn.1001-3776.2019.02.002]
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葱兰夜蛾幼虫肠道细菌群落结构与多样性分析()
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《浙江林业科技》[ISSN:1001-3776/CN:33-1112/S]

卷:
39
期数:
2019年02期
页码:
7-14
栏目:
研究简报
出版日期:
2019-07-05

文章信息/Info

Title:
Analysis of Intestinal Community Structure and Diversity of Larvae of Brithys crini
文章编号:
1001-3776(2019)02-0007-08
作者:
蔡泱莲黄宽官黄俊浩吴 鸿
浙江农林大学 林业与生物技术学院,浙江 杭州 311300
Author(s):
CAI Yang-lianHUANG Kuan-guanHUANG Jun-haoWU Hong
School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China
关键词:
葱兰夜蛾幼虫肠道细菌多样性16S rDNA高通量
Keywords:
Brithys crini larvae intestinal bacteria diversity 16S rDNA high-thoughput sequencing
分类号:
S763
DOI:
10.3969/j.issn.1001-3776.2019.02.002
文献标志码:
A
摘要:
为明确葱兰夜蛾Brithys crini(Fabricius,1775)幼虫肠道细菌的群落结构和多样性,利用IonS5TMXL高通量测序平台对肠道细菌16S rDNA基因V3-V4变异区迚行测序,应用Cutadapt、Uparse等软件整理、统计样品序列数和OUT数,分析肠道细菌的物种组成、丰度和多样性。4个虫体肠道样本测序拼接后,共得273 453条序列和在97%相似度下平均每个样本可聚类为512个OTU数目,总共注释到23门57纲86目168科347属,优势菌群为变形菌门Proteobacteria和厚壁菌门Firmicute。葱兰夜蛾肠道细菌Simpson指数、Shannon指数和Chao1指数分别为0.93 ~ 0.98,5.67 ~ 6.82和439.57 ~ 584.12。结果表明葱兰夜蛾幼虫肠道细菌多样性比较丰富,个体间微生物群落结构和多样性有差异。
Abstract:
To clarify the bacteria community structure and diversity in the intestine of Brithys crini (Fabricius, 1775) larvae, the V3-V4 regions of the 16S rDNA genes of intestinal bacteria in larvae of Brithys crini were sequenced by IonS5TMXL techniques.The numbers of sequences and operational taxonomic units (OTUs), species composition, abundance and diversity of intestinal bacteria were analyzed using Cutadapt and Uparse softwares.A total of 273 453 reads were obtained from the four individuals.Under 97% similarity, each sample could be clustered into 512 OTUs, and annotated into 23 phyla, 57 classes, 86 orders, 168 families and 347 genera.Proteobacteria and Firmicutes were dominated.The diversity indices of Simpson, Shannon and Chao were 0.926-0.975, 5.661-6.823 and 439.565-584.118.The result indicated the high diversity of the intestinal bacteria of B.crini which differed in community structures and diversity among individuals.

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备注/Memo

备注/Memo:
收稿日期:2018-11-05;修回日期:2019-02-19
基金项目:浙江省省院合作林业科技项目(2018SY05)
作者简介:蔡泱莲,硕士研究生,从事昆虫肠道微生物研究;E-mail: 1799982744@qq.com。通信作者:黄俊浩,副教授,从事森林昆虫学研究;E-mail: junhao_huang@hotmail.com。
更新日期/Last Update: 2019-06-20