[1]马晓星,孙伟博,魏辉,等.转PeTLP基因“南林895”杨对土壤微生物的影响及外源基因分子检测[J].浙江林业科技,2018,38(04):28-37.[doi:10.3969/j.issn.1001-3776.2018.04.005]
 MA Xiao-xing,SUN Wei-bo,WEI Hui,et al.Effect of Transgenic Populus deltoides ×P. euramericanna cv. ‘Nanlin 895’ with PeTLP on Soil Microbes and Molecular Analysis on Exogenous Genes[J].Journal of Zhejiang Forestry Science and Technology,2018,38(04):28-37.[doi:10.3969/j.issn.1001-3776.2018.04.005]
点击复制

转PeTLP基因“南林895”杨对土壤微生物的影响及外源基因分子检测()
分享到:

《浙江林业科技》[ISSN:1001-3776/CN:33-1112/S]

卷:
38
期数:
2018年04期
页码:
28-37
栏目:
出版日期:
2018-08-30

文章信息/Info

Title:
Effect of Transgenic Populus deltoides ×P. euramericanna cv. ‘Nanlin 895’ with PeTLP on Soil Microbes and Molecular Analysis on Exogenous Genes
作者:
马晓星1孙伟博1魏辉1刘铃1于翔2王璞1诸葛强1
1. 南京林业大学 南方现代林业协同创新中心,生物与环境学院,江苏 南京 210037; 2. 日本理化学研究所 植物科学中心,横滨 日本 230-0045
Author(s):
MA Xiao-xing1SUN Wei-bo1WEI Hui1LIU Ling1YU Xiang2WANG Pu1ZHUGE Qiang1
1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China; 2. RIKEN Plant Science Center, Yokohama 230-0045, Japan
关键词:
转基因植物‘南林895’杨田间试验生物安全性PeTLP
Keywords:
transgenic plants Populus deltoides ×P. enramericanna cv. ‘Nanlin 895’ field trial biosafety PeTLP
分类号:
S792.11
DOI:
10.3969/j.issn.1001-3776.2018.04.005
文献标志码:
A
摘要:
转基因技术是当今林木分子育种的手段之一,但其生物安全性问题广受关注。本研究选择进入田间试验的 转PeTLP(类甜蛋白)基因‘南林895’杨Populus deltoides× P. euramericana cv. ‘Nanlin 895’为材料,开展转基因 杨树对土壤微生物影响及外源基因分子检测等分析。结果显示,转基因杨树在进入田间试验一年后,外源基因仍 稳定存在于转基因植株基因组中;转基因与非转基因植株根系周围可培养土壤微生物菌落数量无显著差异,表明 外源基因对土壤微生物无显著影响;对土壤微生物总DNA 进行分子检测也显示外源基因未有向周围土壤微生物 扩散现象;叶片化感作用试验显示转基因植株叶片未对生菜Lactuca sativa L. var. ramose Hort.种子的生长造成显 著影响。初步分析结果表明,转PeTLP 基因‘南林895’杨在进入田间试验后未出现外源基因水平转移,也未对周 围生态环境造成显著影响。
Abstract:
Transgenic Populus deltoides× P. euramericana cv. ‘Nanlin 895’ with PeTLP gene were selected for molecular analysis and biosafety analysis. The results showed that the exogenous gene could be detected after one year plantation of transgenic poplars in the open field. There was no significant difference in the quantity of soil microbes around root system between transgenic and non-transgenic plants, indicating that the transgenic plants with PeTLP had no significant effects on soil microbes. Molecular analysis on total DNA of soil microbes showed that the exogenous gene did not transfer to the soil microbes. Allelopathy effect of leaf of transgenic ‘Nanlin 895’ showed that it had no significant effect on the seed growth of Lactuca sativa L. var. ramose Hort.

参考文献/References:

[1] RISHI A S,NELSON N D,GOYAL A,et al. Genetic modification for improvement of Populus[J]. Physiol Mol Biol Plants,2001,7(1):
7-21.
[2] RISHI A S,NELSON N D,GOYAL A,et al. Improvement of Populus through Genetic Engineering[J]. Ind J Plant Physiol,2006(2):119
-126.
[3] 丁莉萍,王宏芝,魏建华. 杨树转基因研究进展及展望[J]. 林业科学研究,2016,29(1):124-132.
[4] 刘潮,韩利红,王海波,等. 植物类甜蛋白基因家族研究进展[J]. 生物技术通报,2018,34(3):9-17.
[5] LOON L C V,PIERPOINT W S,BOLLER T,et al. Recommendations for naming plant pathogenesis-related proteins[J]. Plant Molecular
Biology Reporter,1994,12(3):245-264.
[6] MACKINTOSH CA,LEWIS J,RADMER L E,et al. Overexpression of defense response genes in transgenic wheat enhances resistance to
Fusarium head blight[J]. Plant Cell Rep,2007,26(4):479-488.
[7] RAJAM M V,CHANDOLA N,GOUD P S,et al. Thaumatin gene confers resistance to fungal pathogens as well as tolerance to abiotic stresses
in transgenic tobacco plants[J]. Biologia Plantarum,2007,51(1):135-141.
[8] CHEN W P,CHEN P D,LIU D J,et al. Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express
a rice thaumatin-like protein gene[J]. Theor App Gen,1999,99(5):755-760.
[9] 金红,岳东霞,周良炎,等. 利用类甜蛋白基因诱导表达提高马铃薯对晚疫病的抗性研究[J]. 华北农学报,2001,16(1):67-72.
[10] WANG Q,LI F,ZHANG X,et al. Purification and characterization of a CKTLP protein from Cynanchum komarovii seeds that confers
antifungal activity[J]. PLos One,2011,6(2):e16930.
[11] KALPANA K,MARUTHASALAM S,RAJESH T,et al. Engineering sheath blight resistance in elite indica rice cultivars using genes encoding
defense proteins[J]. Plant Sci,2006,170(2):203-215.
[12] WANG L K,YANG L H,Zhang J,et al. Cloning and characterization of a thaumatin-like protein gene PeTLP in Populus deltoides × P.
euramericana cv.‘Nanlin895’[J]. Acta Physiol Plant,2013,35(10):2985-2998.
[13] 李霞,刘鹏,刘庆,等. 转基因动、植物的研究进展及其安全性分析[J]. 生命科学仪器,2008,6(1):9-13.
[14] HJALTERN J,LINDAU A,WENNSTROM A,et al. Unintentional changes of defence traits in GM trees can influence plant-herbivore
interactions[J]. Basic & Applied Ecology,2007,8(5):434-443.
[15] PRESCOTT V E,HOGAN S P. Genetically modified plants and food hypersensitivity diseases:usage and implications of experimental models
for risk assessment [J]. Pharmacol Therap,2006,111(2):374-383.
[16] 康向阳,刘志明,李胜功,等. 论转基因林木的潜在生态风险性[J]. 应用生态学报,2004,15(7):1281-1284.
[17] 沈孝宙,钱迎倩,张树庸,等. 基因工程树的现状、生态风险与对策[J]. 高技术通讯,2002,12(4):100-105.
[18] JAMES C. 2014 年全球生物技术/转基因作物商业化发展态势[J]. 中国生物工程杂志,2015,35(1):1-14.
[19] 贺熙勇,陈善春,彭爱红. 转基因植物的分子检测与鉴定方法及进展[J]. 热带农业科技,2008,31(1):39-44.
[20] YU X,KIKUCHI A,SHIMAZAKI T,et al. Assessment of the salt tolerance and environmental biosafety of Eucalyptus camaldulensis
harboring a mangrin transgene[J]. J Plant Res,2013,126(1):141-150.
[21] VIAUD M,PASQUIER A,BRYGOO Y,et al. Diversity of soil fungi studied by PCR-RFLP of ITS[J]. Mycol Res,1994,104(9):1027
-1032.
[22] J. 萨姆布鲁克,D.W. 拉塞尔,拉塞尔,等. 分子克隆实验指南[M]. 化学工业出版社,2008.
[23] F UJII Y,PARVEZ S S,PARVEZ M M,et al. Screening of 239 medicinal plant species for allelopathic activity using the sandwich method[J].
Weed Biol Manag,2010,3(4):233-241.
[24] FUJII Y,SHIBUYA T,NAKATANI K,et al. Assessment method for allelopathic effect from leaf litter leachates[J]. Weed Biol Manag,2010,
4(1):19-23.
[25] PERRY J N,TER BRAAK C J,DIXON P M,et al. Statistical aspects of environmental risk assessment of GM plants for effects on non-target
organisms[J]. Environ Biosaf Res,2009,8(2):65-78.
[26] 毛瑢,曾德慧.农林复合系统植物竞争研究进展[J]. 中国生态农业学报,2009,17(2):379-386.
[27] JOSE S,GILLESPIE A R,PALLARDY S G. Interspecific interactions in temperate agroforestry[J]. Agrofor Syst,2004,61-62(1-3):
237-255.[29] 吴锦容,彭少麟. 化感—外来入侵植物的“Novel Weapons”[J]. 生态学报,2005,25(11):3093-3096.
[30] 杨敏生,高宝嘉,王进茂,等. 转双抗虫基因杨基本特性分析[J]. 林业科学,2005,41(1):91-98.
[31] 刘冬艳,张斌,曹杨宇,等. 转抗虫基因杨树外源基因表达的研究进展[J]. 河北林果研究,2015,30(3):243-247.
[32] 魏功峰. 土壤中的微生物[J]. 生物学通报,1995,38(2):48-48.
[33] 李海峰,刘岩,康颖,等. 转基因小黒杨对土壤微生物群落结构的影响[J]. 南京林业大学学报:自然科学版,2014,38(2):75-80.
[34] 朱文旭,丁昌俊,张伟溪,等. 八年生转基因库安托杨外源基因转移及对土壤微生物数量影响的检测[J]. 林业科学研究,2017,30(2):
349-353.
[35] 吕秀华. 转基因银中杨对根际土壤微生物的影响[J]. 基因组学与应用生物学,2018,37(5):1965-1970.
[36] CERDEIRA A L,DUKE S O. The current status and environmental impacts of glyphosate-resistant crop:a review[J]. J Environ Qual,2006,
35(5):1633-1658.
[37] SMALLA K,VAN OVERBEEK L S,PUKALL R,et al. Prevalence of npt II and Tn5 in kanamycin-resistant bacteria from different
environments.[J]. Fems Microbiology Ecology,1993,13(1):47-58.
[28] 万开元,陈防,陶勇,等. 杨树对莴苣的化感作用[J]. 东北林业大学学报,2009,37(1):21-22.

备注/Memo

备注/Memo:
收稿日期:2018-05-15;修回日期:2018-06-22 基金项目:转基因生物新品种培育重大专项(2018ZX08020002);国家林业局生物安全与遗传资源管理项目(KJZXSA2018004);国家 自然科学基金项目(31570650);江苏高校优势学科建设工程项目(PAPD) 作者简介:马晓星,硕士研究生,主要从事分子生物学研究;E-mail:1098130946@qq.com。通信作者:诸葛强,教授,博士生导师,主 要从事杨树遗传改良研究;E-mail:qzhuge@njfu.edu.cn。
更新日期/Last Update: 2018-11-08