[1]施雨彤,徐 梁,郑绍宇,等.千屈菜科中 rpl2 基因内含子缺失现象探析[J].浙江林业科技,2018,38(02):63-68.[doi:10.3969/j.issn.1001-3776.2018.02.010]
 SHI Yu-tong,XU Liang,ZHENG Shao-yu,et al.Loss of rpl2 Intron in Lythraceae[J].Journal of Zhejiang Forestry Science and Technology,2018,38(02):63-68.[doi:10.3969/j.issn.1001-3776.2018.02.010]
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千屈菜科中 rpl2 基因内含子缺失现象探析()
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《浙江林业科技》[ISSN:1001-3776/CN:33-1112/S]

卷:
38
期数:
2018年02期
页码:
63-68
栏目:
试验简报
出版日期:
2018-05-31

文章信息/Info

Title:
Loss of rpl2 Intron in Lythraceae
作者:
施雨彤1徐 梁2郑绍宇1顾翠花1
1. 浙江农林大学 风景园林与建筑学院,浙江 杭州 311300;2. 浙江省林业科学研究院,浙江 杭州 310023
Author(s):
SHI Yu-tong1XU Liang2ZHENG Shao-yu1GU Cui-hua1
1. School of Landscape and Architecture, Zhejiang A & F University, Lin’an 311300, China; 2. Zhejiang Academy of Forestry, Hangzhou 310023, China
关键词:
千屈菜科紫薇属rpl2 内含子内含子缺失
Keywords:
Lythracee Lagerstroemia rpl2 intron intron loss
分类号:
S685.99
DOI:
10.3969/j.issn.1001-3776.2018.02.010
文献标志码:
A
摘要:
本研究共收集了25 种样本,其中紫薇属样本17 种,千屈菜科其它属代表种7 种,并利用近缘物种柳叶菜 科Onagraceae 月见草属Oenothera 植物Oenothera albicaulis 样本作为对照,通过试剂盒法提取DNA,PCR 扩增样 本rpl2 目标区域基因片段,对紫薇属Lagerstroemia Linn 乃至千屈菜科Lythraceae 的rpl2 基因内含子缺失情况进 行了初步研究,探讨rpl2 基因内含子的缺失是否发生在属的水平以及科的水平。实验结果表明,各实验样本的rpl2 目标区域的扩增长度短于非千屈菜科植物,同时通过对扩增片段进行Sanger 测序的结果比对,确认rpl2 基因内含 子缺失现象发生在紫薇属及千屈菜科内。本研究结果对于探讨千屈菜科内植物内含子的缺失机理、紫薇属及千屈 菜科的后续基因组的其他研究提供了前期基础。
Abstract:
Samples of 17 species of Lagerstroemia and 7 other species of Lythracee were collected, and with Oenothera albicaulis of Onagraceae as control. DNA was extracted by the kit method, and the target gene fragment of rpl2 was amplified by PCR. The results showed that the length of the target region of rpl2 was shorter than other species by PCR gel electrophoresis. The comparison of Sanger sequencing of the amplified fragments showed that rpl2 intron loss occurred in tested species except the control.

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备注/Memo

备注/Memo:
收稿日期:2017-09-11 ;修回日期:2018-01-09 基金项目:国家级大学生创新训练项目(201610341017) 作者简介:施雨彤,本科,从事园林植物遗传育种研究;E-mail:305335228@qq.com。通信作者:顾翠花,博士,副教授,从事园林植物 与遗传育种研究;E-mail:gu_cuihua@126.com.
更新日期/Last Update: 2018-06-15