[1]刘亚群,王燕飞,段柳会,等.浙江省无花果种质资源亲缘关系的SRAP 分析评价[J].浙江林业科技,2017,37(05):23-28.[doi:10.3969/j.issn.1001-3776.2017.05.004]
　LIU Ya-qun,WANG Yan-fei,DUAN Liu-hui,et al.SRAP Analysis on Genetic Relationship of Germplasm Resources of Ficus carica in Zhejiang Province[J].Journal of Zhejiang Forestry Science and Technology,2017,37(05):23-28.[doi:10.3969/j.issn.1001-3776.2017.05.004]
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浙江省无花果种质资源亲缘关系的SRAP 分析评价()
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《浙江林业科技》[ISSN:1001-3776/CN:33-1112/S]

卷:
37
期数:
2017年05期
页码:
23-28
栏目:
出版日期:
2017-11-30

文章信息/Info

Title:
SRAP Analysis on Genetic Relationship of Germplasm Resources of Ficus carica in Zhejiang Province
作者:
刘亚群1; 王燕飞2; 段柳会3; 王丽玲1; 张飞英1; 韩素芳1
1. 浙江省林业科学研究院，浙江 杭州 310023；2. 龙泉市林场，浙江 丽水 323700;3. 开化县音坑乡林业站，浙江 衢州 324309
Author(s):
LIU Ya-qun1; WANG Yan-fei2; DUAN Liu-hui3;  WANG Li-ling1; ZHANG Fei-ying1; HAN Su-fang1
１. Zhejiang Academy of Forestry, Hangzhou 310023, China; 2. Longquan Forest Farm of Zhejiang, Lishui 323700, China;3. Forestry Station of Yinkeng Town of Kaihua county, Quzhou, Zhejiang, 324309
关键词:
无花果; 种质资源; 亲缘关系; SRAP; 分子标记
Keywords:
Ficus carica;  germplasm resources;  genetic relationship;  SRAP;  molecular marker
分类号:
S663.3
DOI:
10.3969/j.issn.1001-3776.2017.05.004
文献标志码:
A
摘要:
利用SRAP 分子标记技术，结合物候期对从美国、英国、意大利、法国、中国等地收集的适合浙江省种植的15 个无花果品种的亲缘关系进行分析。结果表明，不同品种物候期存在不同程度差异，萌动期均为3 月中下旬；展叶期在4 月中下旬；座果期基本为5 月初；果实成熟期6 月底、7 月中旬、7 月底，8 月中旬、9 月均有；筛选出SRAP 引物34 个，扩增出204 条多态性条带，多态率为62.25%，表明无花果种质资源的种内变异较为丰富；15 个品种间的遗传相似系数在0.691 ~ 0.922，其中‘波姬红’与‘玛斯义陶芬’，‘美丽亚’与‘金傲芬’之间的遗传相似系数最大，为0.922，亲缘关系最近；‘红矮生’和‘日本紫果’遗传相似系数最小，为0.691。聚类分析表明在遗传距离为0.735 处，不同品种分为两个大类群，其中‘红矮生’单独聚为一类，其余品种聚为一类。物候期观察表明，同一生长环境下，物候期与品种亲缘关系相关。
Abstract:
Fifteen culitvars of Ficus carica from the United States, the United Kingdom, Italy, France and China were planted in Wuyi, Zhejiangprovince in 2015, leaves were collected in March 2016 for evaluation of genetic relationships among these cultivars by SRAP markers andobservations on phenological periods. The results showed that there were differences in phenological period among 15 germplasm resources, withsprouting period from middle to late March, leaf expansion from middle to late April, fruit setting at early May, and fruit mature from late June to theearly September. 34 SRAP primers were selected, 204 polymorphic bands were obtained by PCR with the polymorphic rate of 62.25%, indicatingabundant intraspecific variation. The genetic similarity coefficient among 15 cultivars was 0.691-0.922, with the maximal coefficient of 0.922 of‘A132’ and ‘Masui Dauphine’, and the minimal of 0.691 of ‘Violette Solise’ and ‘Hongaisheng’. Cluster analysis showed that tested germplasm couldbe divided into two groups at genetic distance of 0.735. ‘Hongaisheng’ was clustered alone, the others were clustered into one group. Observation onphenological period of tested cultivars planted in the same location showed that their phenology had relation with their provenances..

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备注/Memo

备注/Memo:
收稿日期：2017-03-25 ；修回日期：2017-07-08基金项目：浙江省科研院所专项（2015F30002）作者简介：刘亚群，高级工程师，从事林业土壤、植物的分析检测工作；E-mail：liuyaqun2005@aliyun.com。通信作者：韩素芳，副研究员，从事经济林栽培研究；E-mail：hansufang2004@126.com。
更新日期/Last Update: 2017-10-20