[1]李皓,董建华,董建明,等.退化与正常山核桃林土壤细菌多样性比较研究[J].浙江林业科技,2017,37(05):42-47.[doi:10.3969/j.issn.1001-3776.2017.05.007]
　LI Hao,DONG Jian-hua,DONG Jian-ming,et al.Comparison on Soil Bacteria Diversity between Degrated and Healthy Carya cathayensis Forests[J].Journal of Zhejiang Forestry Science and Technology,2017,37(05):42-47.[doi:10.3969/j.issn.1001-3776.2017.05.007]
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退化与正常山核桃林土壤细菌多样性比较研究()
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《浙江林业科技》[ISSN:1001-3776/CN:33-1112/S]

卷:
37
期数:
2017年05期
页码:
42-47
栏目:
出版日期:
2017-11-30

文章信息/Info

Title:
Comparison on Soil Bacteria Diversity between Degrated and Healthy Carya cathayensis Forests
作者:
李皓1; 董建华1; 董建明2; 袁紫倩1; 胡俊靖1; 赵伟明1
1. 杭州市林业科学研究院，浙江 杭州 310022；2. 杭州市临安区林业局，浙江 杭州 311300
Author(s):
LI Hao1; DONG Jian-hua1; DONG Jian-ming2; YUAN Zi-qian1; HU Jun-jing1; ZHAO Wei-ming1
1. Hangzhou Forestry Academy of Zhejiang, Hangzhou 310022, China; 2. Lin’an Forestry Bureau of Zhejiang, Hangzhou 311300, China
关键词:
山核桃; 退化; 土壤细菌群落; 16SrDNA 测序; 多样性
Keywords:
Carya cathayensis;  degraded;  soil bacteria community;  16SrDNA sequencing;  diversity
分类号:
S664.1
DOI:
10.3969/j.issn.1001-3776.2017.05.007
文献标志码:
A
摘要:
2014 年10 月，在浙江省临安市选择4 个退化山核桃Carya cathayensis 林样地和2 个正常山核桃林样地，采土样后用16SrDNA 测序技术分析两种林地土壤细菌多样性。结果表明，6 个样地土壤细菌共有36 门75 纲96目224 科924 属。在门的水平上，退化和正常林地细菌主要类群基本一致，主要类群有变形菌门Proteobacteria，酸杆菌门Acidobacteria，拟杆菌门Bacteroidetes，绿弯菌门Chloroflexi，厚壁菌门Firmicutes，疣微菌门Verrucomicrobia，放线菌门Actinobacteria 和浮霉菌门Planctomycetes，但不同林地中各类群相对丰度明显不同；在属的水平上，退化和正常林地差别很大。Alpha 多样性分析显示，6 个样地Shannon 指数，ACE 指数，Chao1指数的排序各不相同，分析说明山核桃林退化程度与土壤细菌数量和多样性无关。相关分析结果表明，ACE 指数与交换性H+显著相关，其余土壤物理化学性质与细菌多样性指数均无显著相关性。
Abstract:
Four sample plots in degraded Carya cathayensis stand and 2 in normal ones were established in October 2014 in Lin’an district, Zhejiangprovince. Soil samples were sequenced by 16SrDNA for comparison on bacteria community diversity of different sample plots. The results indicatedthat there were 924 genera of 224 families and 36 phylums, 75 classes, 96 orders in sampled plots. The main phylums were the same in the testedplots, included Proteobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Verrucomicrobia, Actinobacteria and Planctomycetesbut withdifferent relative abundance . The distribution of main genera had great difference between degraded and normal plots. The Shannon index, the ACEindex and Chao1 index showed that it had no relationship between degradation of sampled stands with bacteria richness and diversity. Correlationanalysis demonstrated that ACE index had evident relation with H+, the other physiochemical properties of the tested had no significant correlationwith bacteria diversity index.

参考文献/References:

[1] Fierer N，Jackson R B. The diversity and biogeography of soil bacterial communities[J]. Proceed Natl Acad Sci U S A，2006，103（3）：626－631．
[2] Chen Q H，Feng Y，Zhang Y P，et.al. Short-term responses of nitrogen mineralization and microbial community to moisture regimes ingreenhouse vegetable soils[J]. 土壤圈（英文版），2012，22（2）：263－272．
[3] 李桥. 基于高通量测序技术下土壤微生物群落结构的研究[D]. 济南：山东师范大学，2011．
[4] 曾希柏，王亚男，王玉忠，等. 不同施肥模式对设施菜地细菌群落结构及丰度的影响[J]. 中国农业科学，2013，46（1）：69－79．
[5] 张彩霞. 新一代高通量测序技术研究土壤微生物群落结构对环境条件的响应[D]. 南京：南京农业大学，2012．
[6] 秦楠，栗东芳，杨瑞馥. 高通量测序技术及其在微生物学研究中的应用[J]. 微生物学报，2011，51（4）：445－457．
[7] 宋素灵. 山核桃林地土壤退化现状和施肥改良研究[D]. 临安：浙江农林大学，2014．
[8] 杨菁，周国英，田媛嫒，等. 降香黄檀不同混交林土壤细菌多样性差异分析[J]. 生态学报，2015，35（24）：8117－8127．
[9] 常显波，刘举，等. 不同种植年限苹果园土壤理化性质及微生物数量[J]. 安徽农业科学，2007，35（5）：1423－1426．
[10] 黄珍. 海南福山香蕉地土壤细菌和真菌多样性分析[D]. 海南：海南大学，2010.
[11] Peck G M，Merwin I A，Thies J E，et.al. Soil properties change during the transition to integrated and organic apple production in a New Yorkorchard[J]. App Soil Ecol，2011，48：18－30.
[12] 贾丽. 珍稀濒危植物白皮松土壤微生物多样性研究[D]. 兰州：西北师范大学，2007.

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备注/Memo

备注/Memo:
收稿日期：2017-03-21；修回日期：2017-08-13基金项目：浙江省林业科研成果推广项目（2013B13）作者简介：李皓，工程师，从事经济林研究；E-mail：38761202@qq.com。
更新日期/Last Update: 2017-10-20